Data/hora: 01/06/2024 15:42:18

Provedor de dados: | Genetics and Molecular Biology
País: | Brazil
Título: | Comparative cytogenetic studies of Bufo ictericus, B. paracnemis (Amphibia, Anura) and an intermediate form in sympatry
Autores: | Azevedo,MFC; Foresti,F.; Ramos,PRR; Jim,J.
Data: | 2003-01-01
Ano: | 2003
Palavras-chave: | Bufo; Hybridization; Cytogenetics; Nuclear DNA; Seroproteins.
Resumo: | Specimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a remarkable difference in the patterns of the protein bands whose molecular weight corresponded to that of albumin. While the parental species presented two different bands, the intermediate form presented 4. However, only three of these bands were seen in each specimen. The results obtained pointed to a high probability for natural hybridization between Bufo ictericus and Bufo paracnemis in the site and specimens studied.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300012
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572003000300012
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.26 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess